Each detergent–lipid combination was prepared at seven different q ratios: 0.5, 1, 1.5, 2, 2.5, 3, and 5 by resuspending lipid/detergent films in a buffer containing the detergent of choice at a concentration slightly above the critical micelle concentration (CMC) and subjecting them to four freeze–thawing cycles to induce bicelle formation prior to structural analysis (see the methods section for details). For each of the three detergents, bicelles were prepared with four lipid compositions: (i) saturated zwitterionic (DMPC), (ii) saturated zwitterionic/anionic (DMPC:DMPG 80:20), (iii) unsaturated zwitterionic (POPC), and (iv) unsaturated zwitterionic/anionic (POPC:POPG 80:20) ( Figure 1).
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